Results In suspension (without protein) aqueous solutions (dilutions) partly show bactericidal, tuberculocidal and fungicidal effects. But when adding a 20% cattle serum a microbicidal effect fails to appear even with high concentrations of the preparation (see Table 1). Table 1: Bactericidal effect of Stalosan F in suspension test (with and without protein loading, 20°C. Test bacterium Staph. Aureus Stalosan conc. (%) 1% phenole 0 0,5 1 2 4 8 16 32 Without serum Affecting time (min) 5 15 30 60 - +++ ++ + + + ++ ai.- ai.- ai.- ai.+ ai.- ai.- ai.- ai.+ ai.- ai.- ai.- ai.+ ai.- ai.- ai.- ai.+ ai.- ai.- ai.- ai.+ ai.- ai.- ai.- With serum Affecting time (min) 5 15 30 60 +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ ++ +++ ++ ++ + ++ ++ ++ + ++ ++ ++ + Ent. feacium Legend: 1% phenole 0 0,5 1 2 4 8 16 32 +++ strong growth ++ middle growth + visible growth - +++ ++ + + + ++ ai.+ ai.- ai.- ++ ai.- ai.- ai.- ai.+ ai.- ai.- ai.- ai.+ ai.- ai.- ai.- ai.- ai.- ai.- ai.- ai.- ai.- ai.- ai.- +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ ++ ++ ++ ++ ai.+ growth after incubation on medium ai.- no growth after incubation on medium It is true that "Stalosan F" dilutions show no or only a very limited cytotoxic and virus-deactivating effect but cause a clear reduction of the virus titre of about 4 up to 5 lg in the suspension tests and 1,5 upo to 3 lg in the germ carrier tests (Tab.2). Table 2: Virucidal effect (reduction of titer) of 5% and 1% Stalosan dilutions in suspensions test. Test virus ECBO REO Vaccinia Newcastle Disease Stalosan Conc. (%) 5 1 5 1 5 1 5 1 Reduction of titer (log 10 KID50) * After different affecting times (min) 0 15 30 45 60 6,75 3 2,92 2,92 2,83 6,75 3,58 3,58 3,5 3 6,42 2,83 3 2,83 2,58 6,42 3,17 3,25 2,83 3 7,25 3,42 3,33 3,17 3,17 7,25 3,42 3,5 3,33 3,25 7,67 3,67 3,58 3,42 3,42 7,67 4 3,83 3,66 3,5 * mean values of 3 tests
Download PDF file