STALOSAN F - Not a chemical disinfectant, but a very good hygiene substance W. Methling 1 , Regina Dibbert 1 , H. - W. Heinrich 2 , U. Meyer 2 , (1) University of Rostock, Department of Agriecology, Institute of Ecologically-Freindly Husbandry, 18051 Rostock, Germany (2) Privates Institute Bioserv, Dr.-Lorenz-Weg 1, 18059 Rostock, Germany. Summary The Danish preparation "Stalosan F" was tested on the basis of guides for testing of didinfectants issued by the German Veterinary Society. "Stalosan F" is to be applied as powder, but the test method is intended an suitable only for the examination of soluble chemical disinfectants. Therefore we test also the effect of "Stalosan F" powder on contaminated wooden carriers. "Stalosan F" has no or low bactericidal, tuberculocidal, fungicidal and virucidal effects. But there is a very strong reduction (4-5 lg) of virus titer, probably also a reduction of a number of bacteria. The decreasing effect on microbial contamination of surfaces caused by adsorption of microorganisms, the binding of water and urea on floor, the reduction of ammonium emission and a little deodorization effect qualify "Stalosan F" as a very good hygiene substance. Keywords: disinfectant, testing of disinfectant, Stalosan F, hygiene substance Introduction According to reports from practice the preparation "Stalosan F" produced in Denmark (by comp. Stormøllen) shows very goods effects on the animal health and envinronment when scattering it as a powder on animal house surfaces. The producer considers "Stalosan F" to be a disinfectant. In Germany tested disinfectants are included into an offical llist and by this are recommended for application if their microbicidal effect has been proved true according to the Guidlines for the Examination of Chemical Disinfectants issued by the Disinfection Commission of the German Veterinary Society (DVG, 1988) Material and methods "Stalosan F" is a brown-red powder which mainly consists of phosphate compounds (85%), Ca-, and Fe- sulphates (2,6%), active chlorine (0,25%), Perica oil (0,05%) and Al-silicate (10,1 %). It is intended for the application as powder (up to 50 g per sqm). The test method prescribed by DVG refers only to aqueous solutions of chemical disinfectants. Therefore besides the official methods for the ezamination of aqueous solutions a modified test procedure had to be applied to the powdery "Stalosan F". The examination of the microbocidal effect of the preparation (pH value 3,45 up to 2,41) was performed by suspension and germ carrier tests with the following strains: - Staphylococcus aureus (ATTC 6538) - Enterococcus faecium (Kulmbach Str. 2) - Proteus mirabilis (ATCC 14153) - Pseudomonas aeruginosa (AtCC 15442) - Mycobacterium avium (Av 56) - Candida albicans (ATCC) Appropriate deactivating agents were 3% Tween 80 (for gram-positive bacteria and Candida), 0,5% Na-thio- sulphate (for gram-negative germs) and 0,1% Cysteine (for Mycobacterium). After pretests for toxicity and suspension tests had been performed the virucidal effect of 1% up to 5% dilutions of "Stalosan F" was examined by germ carrier tests (on mull and wood) with following strains: - ECBO virus (LCR-4) - REO virus (type 1) - Newcastle Disease virus (Montana) - Vaccinia virus (Orthopoxvirus commune, Elstree)
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