- Escherichia coli (E. coli), Salmonella sp (Sal. sp), Staphylococcus sp (Sta. sp) and Streptococcus sp (Strep.Sp) with a minimum concentration at 10 9 cfu/ml; - ASF, FMD, PRRS and Influenza A/H5 viruses with a minimum concentration at 10 6 TCID50/ml; - Types of agar and cell culture medium suitable for each type of bacteria and virus; - Other necessary materials. 4. Test method: - Preparation of bacteria and viruses mixed in PBS solution according to the concentration required in the test plan; - Prepare 01 clean cage. Put the opened-empty Petri in the spray area. Spraying Stalosan F disinfectant on the surface of the cage, including the Petri dish surface with a density of 50g/m2 with a specialized sprayer; for each type of pathogen, proceed according to the following steps: + Immediately after spraying, use 2 Petri dishes containing disinfectant, drop 1 ml of pathogen liquid into each dish. Wait 10 minutes for disc 1 and 30 minutes for disc 2. Then use 9 ml of EMEM + 5%FBS to pour into each dish, retracted into the sample tube. Shake the tube well and centrifuge at 1500 rpm for 5 minutes. Transfer the supernatant to another tube. Adjust the pH to 7, if necessary. + Each sample after harvesting is diluted in EMEM + 5% FBS base 10 into suspensions with a concentration of 10 0 to 10 -4 (5 samples) for survival microbial titration. Comparison with the initial microbial density to assess the pathogen inactivation effect of the disinfectant. * At the time of 3 days and 7 days after spraying, repeat the same experiment to evaluate the prolonged effect of the drug at such times. -With other pathogens, follow the same procedure. 2
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