Table 3. Effect of Stalosan F powder on TGEV (Experiment 3) Dose of the sanitizer Time of contact Virus Titer (log 10 TCID 50 /0.1 ml) in: Control Stalosan F Percent virus reduction 5 min 4.5 <0.5 ≥ 99.99 10 min 4.5 <0.5 ≥ 99.99 A 1 h 4.5 <0.5 ≥ 99.99 6 h 3.83 <0.5 ≥ 99.95 5 min 4.5 <0.5 ≥ 99.99 10 min 4.5 <0.5 ≥ 99.99 B 1 h 4.5 <0.5 ≥ 99.99 6 h 3.83 <0.5 ≥ 99.95 A: 10 mg Stalosan F powder Control B: 20 mg Stalosan F powder Control TCID 50 : 50% tissue culture infective dose of virus A: 10 mg beef extract powder B: 20 mg beef extract powder Conclusion: Stalosan F powder, when applied at 10mg/2cm 2 and 20mg/2cm 2 surface area, was able to inactivate ≥99.9 9% of TGEV within 5 minutes. 5 Virus titer (log 10 TCID 50 /0.1ml) 4 3 control 2 Stalosan F 1 0 5 min 10 min 1 h 6 h Figure 1: Effect of Stalosan F powder on TEGV at a dose of 10 mg/ 2cm 2 4
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