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Technical Report CH – 691/2016 INTRODUCTION The Test Facility conducted a study to evaluate the antimicrobial activity of two formulation of Stalosan F dry disinfectant against Salmonella thyphimurium , according to the protocol supplied by the sponsor. The study was performed in compliance with the following guidelines: - EN 13697:2015 “Chemical disinfectants and antiseptics - Quantitative non porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas – Test method and requirements without mechanical action (phase 2/step 2). and the following data requirements: Regulation (EU) No 528/2012 of the European Parliament and of the Council of 22 May 2012 concerning the making available on the market and use of biocidal products. The experimental phase of this study started on November 7, 2016 and was completed on November 10, 2016. EQUIPMENT - Autoclave, HV-85L, Internal code No. 574 - Bath, GRANT SUB 14, Internal code No. 226 - Thermostatic oven, BIOLOG AG-System, Internal code No. 693 - Thermostatic oven, BIOLOG AG-System, Internal code No. 612 - Thermostatic oven, MPM 90, Internal code No. 310 - Vortex mixer - Laminar flow sterile cabinet STERIL/BH, Class II Biohazard, Internal code No. 651 - Usual microbiology sterile materials - Microscope, LEICA, Internal code No. 212 - Micropipettes with variable volume REAGENTS - Tryptone Soya Agar (TSA) (Merck) - Maximum recovery diluent (Merck) - Lecithin, reagent grade (Merck) - Polysorbate 80 (Merck) - L-Histidine, reagent grade (Sigma) - Tryptone (Fluka) - Yeast extract (Fluka) page 6 of 16
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Technical Report CH – 691/2016 EXPERIMENTAL Principle of the method A test suspension of bacteria in a solution of interfering substances is inoculated onto a carrier paper surface and dried. A prepared sample of the test item is applied in order to cover the dried film. The surface is maintained at a specified temperature for a defined time. The surface is transferred to a previously validated neutralization medium so that the action of the disinfectant is immediately neutralized. The number of surviving organisms which can be recovered from the surface is determined quantitatively. Safety Precaution Each analyst should be acquainted with potential hazards of the reagents, products and solvents before commencing laboratory work. SOURCES OF INFORMATION INCLUDE: MATERIAL SAFETY DATA SHEETS, LITERATURE AND OTHER INTERNALLY- GENERATED DATA. Safety information on products should be requested from the supplier. Disposal of reagents, reactants and solvents must be in compliance with the appropriate government regulations. Reference Materials Salmonella typhimurium ATCC 14028 page 7 of 16
