Results: 1. Surface test was performed using 10 mg and 20 mg of Stalosan powder with four different contact times (5, 10, and 60 minutes and 6 hours). For each experiment the inoculated cells were washed twice with HANKS solution after 90 minutes of incubation with different dilutions of the samples, and then followed by incubation at 37°C. This approach was successful to avoid the cytotoxic effect of the sanitizer itself on CEF cells . The 90 min was chosen because it enough for the survived virus to attach with the hosting cells if it presents. 2. This experiment was done in duplicate and the results are shown in Tables 1 and 2. Table 1. Effect of Stalosan F powder on IBDV (Experiment 1) Dose of the sanitizer Time of contact Virus Titer (log 10 TCID 50 /0.1 ml) in: Percent virus reduction Control Test 5 min 4.5 3.17 95.323 10 min 4.5 2.17 99.532 A 1 h 4.17 <0.5 ≥ 99.979 6 h 3.83 <0.5 ≥ 99.953 5 min 4.5 1.17 99.953 10 min 4.5 1.17 99.953 B 1 h 4.5 <0.5 ≥ 99.990 6 h 3.83 <0.5 ≥ 99.953 A (Test): 10 mg Stalosan F powderl A (Control): 10 mg beef extract powder B (Test): 20 mg Stalosan F powder B (Control): 20 mg beef extract powder TCID 50 : 50% tissue culture infective dose of virus Table 2. Effect of Stalosan F powder on IBDV (Experiment 2) Virus Titer Dose of the sanitizer Time of contact (log 10 TCID 50 /0.1 ml) in: Control Test Percent virus reduction 5 min 4.17 3.17 90.000 A 10 min 4.5 2.5 99.000 1 h 4.17 <0.5 ≥ 99.979 6 h 3.5 <0.5 ≥ 99.900 5 min 4.5 1.5 99.900 B 10 min 4.17 1.17 99.900 1 h 4.5 <0.5 ≥ 99.990 6 h 3.83 <0.5 ≥ 99.953 A (Test): 10 mg Stalosan F powder A (Control): 10 mg beef extract powder B (Test): 20 mg Stalosan F powder B (Control): 20 mg beef extract powder TCID 50 : 50% tissue culture infective dose of virus 3
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