TABLE OF CONTENTS SCHEDULE 1 4 OUTLINE PROTOCOL FOR TRIAL TO EVALUATE THE EFFICACY OF TEST DISINFECTANT AGAINST COCCIDIAL OOCYSTS. 4 Test material 4 Results and report 6 MATERIALS AND METHODS 7 Test material 7 Preparation of parasites 7 Stage 1. Exposure of unsporulated oocysts 7 Stage 2. Exposure of sporulated oocysts. 8 RESULTS 10 Stage 1. Exposure of unsporulated oocysts 10 Effect of Stalosan F on the sporulation rate of coccidial oocysts 10 Stage 2. Exposure of sporulated oocysts. 11 Effects on growth rate of chicks 11 CONCLUSIONS 13 Effects of Stalosan F disinfectant on unsporulated oocysts. 13 Effects of Stalosan F disinfectant on sporulated oocysts. 13 Appendix 1 - Treatment of unsporulated oocysts 14 Sporulation counts of oocysts treated before sporulation 14 Appendix 2 - Treatment of sporulated oocysts 15 Counts of sporulated oocyst cultures 15 Raw data bodyweights for infected chicks 15 Faecal oocyst counts from chicks 15
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