After the required exposure time the eggs were examined and counted. It was only possible to recover between 126 and 315 of the initially incubated 750 parasite eggs. Thus, between 58 % and 83 % of the eggs were lost during the incubation time or during the procedure where the eggs were washed free of disinfectant or sand. On average, 179 eggs, i.e. ~24 % of the total number of incubated eggs were recovered in the Stalosan F - treated groups. In the control groups, an average of 231 eggs, i.e. ~31 % of the incubated eggs were recovered. However, the difference in recovery of eggs was not insignificant (P > 0.05) in all four parasite egg batches. In the following, the results are presented as normal appearing eggs as a percentage of the total number of recovered eggs. Fig. 2 Normal appearing eggs as a percentage of the total number of re-found eggs after 1, 2, 3, 7 or 21 days of in vitro exposure to Stalosan F or commercial bird sand (control). A batch of embryonated (L 3 stage) mixed eggs of A. galli , H. gallinarum and Capillaria spp. was used As shown in Fig. 1-4, the proportion of normal appearing eggs was between 25 % and 50 % of the total number of recovered eggs, i.e. between 50 % and 75 % of the total number of recovered eggs were either dead or abnormal. The proportion of normal appearing eggs was very similar in both the Stalosan F -treated and in the control group at most examination times in all four parasite egg batches. Statistically significant difference (P < 0.05) between the Stalosan F - treated and the control group was only found at one occasion: In the batch of embryonated A. galli eggs originally extracted from the uteri of female worms, significant more normal appearing eggs were found in the Stalosan F - treated than in the sandtreated control group (Fig. 4). Fig. 3. Normal appearing eggs as a percentage of the total number of recovered eggs after 1, 2, 3, 7 or 21 days of in vitro exposure to Stalosan F or commercial bird sand (control). The egg batch consisted non-embryonate d A. galli eggs freshly extracted from the uteri of female worms .
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