Experiment 3: Natural infection study A pen, naturally contaminated with A. galli eggs, was divided into two smaller but equally sized pens. In one pen Stalosan F was applied as recommended by the producer, i.e. 50 g/m 2 for three consecutive days and then every week. The other pen was left as an untreated control. Twenty-five parasite-free chickens, two weeks of age, were introduced into each pen. The chickens had free access to water and feed, and their weight was recorded at week 1, 3, 5 and 6 after the introduction to the pens. All animals were slaughtered at week 7, and the intestines were examined for the presence of adult, as well as larval stages of A. galli . Statistical analysis Differences in means of egg excretion between the different groups of chickens were analysed for each observation time using the unpaired t-test with a 95 % confidence limit in the data processing software GraphPad Prism®. Worm burdens were similarly compared between the different groups. Results Experiment 1: in vitro experiments Fig. 1. Normal appearing eggs as a percentage of the total number of re-found eggs after 1, 2, 3, 7 or 21 days of in vitro exposure to Stalosan F or commercial bird sand (control). A batch of newly excreted non-embryonated mixed eggs o f A. galli , H. gallinarum and Capillaria spp. was used.
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