Table 1. Trial design of the in vitro study to evaluate the effect of Stalosan F on four different batches of parasite eggs Group Powder Parasite eggs Time Newly excreted (non-embryonated) mixed eggs a 1 Stalosan 24h 2 Sand 24h 3 Stalosan 48h 4 Sand 48h 5 Stalosan 72h 6 Sand 72h 7 Stalosan 1 week 8 Sand 1 week 9 Stalosan 3 weeks 10 Sand 3 weeks Embryonated (L 3 stage) mixed eggs a 11 Stalosan 24h 12 Sand 24h 13 Stalosan 48h 14 Sand 48h 15 Stalosan 72h 16 Sand 72h 17 Stalosan 1 week 18 Sand 1 week 19 Stalosan 3 weeks 20 Sand 3 weeks Freshly extracted (non-embryonated) A. galli eggs b 21 Stalosan 24h 22 Sand 24h 23 Stalosan 48h 24 Sand 48h 25 Stalosan 72h 26 Sand 72h 27 Stalosan 1 week 28 Sand 1 week 29 Stalosan 3 weeks 30 Sand 3 weeks Embryonated (L 3 stage) B. galli eggs b 31 Stalosan 24h 32 Sand 24h 33 Stalosan 48h 34 Sand 48h 35 Stalosan 72h 36 Sand 72h 37 Stalosan 1 week 38 Sand 1 week 39 Stalosan 3 weeks 40 Sand 3 weeks a consisted mainly of A. galli, H. gallinarum and Capillaria spp. eggs; b extracted from the uteri of female worms
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