2012 AASV Annual Meeting: Integrating Science, Welfare, and Economics in Practice Figure 2: Figure 3: and tested by PCR to confirm the presence of the virus (12/trt). Inocu- lated boots were consecutively placed in each boot bath per treatment group, and the bottoms of the boots were Swiffer sampled and tested by PCR 1 min post-disinfection. This trial (with and without fecal matter) was then repeated with sampling after 3 min. and 5 min post-disin- fection. A sample of each boot bath was taken at the completion of each treatment group (12/ trt) and tested by PCR. In Trial 2, the environmental temper- ature was 8°F (a temperature below the freezing point of Synergize). The same method used in Trial 1 was re- peated at this temperature; 1, 3 and 5 minutes post-disinfection; and with and without the presence of fecal matter on the bottom of the boot. Synergize was frozen at this tempera- ture and unable to be tested. Trial 3 was conducted in field con- ditions to demonstrate the use of Stalosan F powder baths in summer conditions and in the presence of fecal matter. A single flow weaning PRRSv negative piglets was used. Three nursery sites receiving piglets daily from this flow were sampled 5 days per week over an 8 week period of time as they received piglets. Pig- lets were vaccinated with Ingelvac PRRS MLV vaccine upon arrival to the nursery sites. Stalosan F powder boot baths were placed at the entry to the site and at the entries to each barn. Site protocol required the workers to step into the boot baths upon entry and exit to each barn. Thirty of the first piglets to arrive at the nursery site were bled weekly be- ginning one week post placement to confirm vaccine virus infection and shedding. The bottoms of a worker’s boots were Swiffer sampled daily, and tested by PCR. 98 American Association of Swine Veterinarians
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