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- DLF Forage Seeds White Paper - Festulolium Hybrid Grass Festulolium is the name for a hybrid forage grass developed by crossing Meadow Fescue (Festuca pratense) or Tall Fescue (Festuca arundinacea) with perennial ryegrass (Lolium perenne) or Italian ryegrass (Lolium multiflorum). This enables combining the best properties of the two types of grass. The resulting hybrids have been classified as: Maternal parent Paternal parent Hybrid progeny progeny or back crossing the hybrid progeny to its parental lines, a wide range of varieties with varying characteristics and phenotypes has been created. They are classified according to their degree of phenotypical similarity to the original parents, not to their genotype heritage. One can regard them as high yielding fescues with improved forage quality or as high yielding, more persistent ryegrasses. This genotype make-up of festuloliums can be made visual. The chromosomes of festulolium can be isolated and then colored to show the parental origin of chromosome sections. It provides a very visual effect of the hybridization between the two species. Photo right: Chromosomes of a festulolium, colored to show the parental DNA in the hybrid. Green = Ryegrass DNA Red = Fescue DNA Festuca arundinacea Festuca arundinacea Festuca pratensis Festuca pratensis Lolium multiflorum Lolium perenne Lolium multiflorum Lolium perenne Festulolium pabulare Festulolium holmbergii Festulolium braunii Festulolium loliaceum The fescues contribute qualities such as high dry matter yield, resistance to cold, drought tolerance and persistence, while ryegrass is characterized by rapid establishment, good spring growth, good digestibility, sugar content and palatability. The individual festulolium varieties contain various combinations of these qualities, but all are substantially higher yielding than their parent lines. While festuloliums have been around for many years, the true potential had never been pursued in earnest. DLF has developed a substantial breeding program in hybrid festulolium that has produced a unique range of hybrid festulolium varieties. After initial hybridization and subsequent selection on the hybrid Via a newly developed marker analysis, Diversity Array Technology (DArT), the actual percentage of parental DNA can be determined and the characteristics of a newly made hybrid can be fairly well predicted. This makes the selection process after initial hybridization more efficient and much shorter. % 100 Lo liu m mu oru ltifl m Festulolium DiamondTM Perseus 100% F tuca p es Becva Hostyn Perun Lofa Felina, Mahulena % Fest a uca run Festulolium Diamond Explained ned ows the relationship The Festulolium Diamond shows nd the morphological between the genetic makeup and ulolium varieties. More blue expression of the various festulolium means more ryegrass genetics cs and thus more ryegrass characteristics. Within the blue section, there is s transgression from Lolium multiflorum to Lolium hybridum to Lolium perenne. renne. The less blue, the more genetic background from either fescue parent and associated morphological expression. Hykor m oliu pe 100% L DLF International Seeds . PO Box 229 Halsey . OR 97348 USA tel: 541.369.2251 . toll free: 800.445.2251 . e-mail: : info@dlfis.com 1 00 ensi rat s renne dinacea Fojtan

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